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![]() Each containing different enhancers to increase signal, linearity and duration compared to other western blot detection systems. We offer six types of ECL substrates for western blot detection with horseradish peroxidase enzyme (HRP). Light emission occurs only during the enzyme-substrate reaction therefore, once the substrate in proximity to the enzyme is exhausted, signal output ceases. When incubated with a blot on which HRP-conjugated antibodies (or other probes) are bound, a chemical reaction emits light at 425 nm which can be captured with x-ray film, CCD camera imaging devices and phosphorimagers that detect chemiluminescence. To make a working solution, the two components are mixed together. Same signal and lower price than other standard ECL substratesĮnhanced chemiluminescence (ECL) substrates, are HRP substrates that are typically two-component systems consisting of a stable peroxide solution and an enhanced luminol solution. Good sensitivity with long duration and linearity for sensitive quantitationīetter signal and lower price than competing ECL substrates Highest sensitivity with less optimization required Performing quantitative western blots or long duration is neededįor routine western blots or new systems not yet optimizedĭetecting high abundant protein targets and sample is abundant Detecting very low abundant protein targets, limited antibodies, or limited sample volumes
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